The Lee Laboratory uses emerging biotechnologies to discover and design new approaches to cardiovascular diseases. A central theme of our laboratory is that merging bioengineering and molecular biology approaches can yield novel approaches. Thus, we work at this interface using a broad variety of techniques in genomics, imaging, nanotechnology, physiology, cell biology, and molecular biology. Our approach is to understand problems and design solutions in the laboratory and then demonstrate the effectiveness of these solutions in vivo. Ongoing projects in the laboratory include studies of cardiac regeneration, diabetic vascular disease, wound healing, heart failure, and cardiac hypertrophy.
Recent Publications
Senyo SE, Steinhauser ML, Pizzimenti CL, Yang VK, Cai L, Wang M, Wu TD, Guerquin-Kern JL, Lechene CP, Lee RT. Mammalian Heart Renewal by Preexisting Cardiomyocytes. Nature 2012; in press.
Gottumukkala, RV, Cornivelli L, Wagers AJ, Kwong RY, Bronson R, Stewart GC, Schulze PC, Chutkow W, Wolpert HA, Lee RT, Lipes, MA. Myocardial Infarction Triggers Chronic Cardiac Autoimmunity in Type 1 Diabetes. Science Translational Medicine 2012;4:138.
Kakkar R, Hei, H, Dobner S, Lee RT. Interleukin 33 as a Mechanically Responsive Cytokine Secreted by Living Cells. J Biol Chem 2012;287:6941-6948.
Steinhauser ML, Bailey A, Senyo SE, Guillermier C, Perlstein TS, Gould AP, Lee RT, Lechene CP. Quantitating subcellular metabolism with multi-isotope imaging mass spectrometry. Nature 2012;481:516-9.
Yoshioka J, Chutkow WA, Lee S, Kim JB, Yan J, Tian R, Lindsey ML, Feener EP, Seidman CE, Seidman JG, Lee RT. Deletion of Thioredoxin-Interacting Protein Impairs Mitochondrial Function but Protects the Myocardium from Ischemia-Reperfusion Injury. J Clin Invest 2012;122:267-79.
Patwari P, Emilsson V, Schadt EE, Chutkow WA, Lee S, Marsili A, Zhang Y, Dobrin R, Cohen DE, Larsen PR, Zavacki AM, Fong LG, Young SG, Lee RT. The Arrestin Domain Containing 3 (ARRDC3) Protein Regulates Body Mass and Energy Expenditure. Cell Metabolism 2011; 14:671-83.
Kanki S*, Segers VFM*, Wu W, Kakkar R, Gannon J, Sys SU, Sandrasagra A, Lee RT. Stromal Cell-derived Factor-1 Retention and Cardioprotection for Ischemic Myocardium. Circulation: Heart Failure 2011; 4:509-18. *Equal contributors.
Chutkow WA, Lee RT. Thioredoxin regulates adipogenesis through thioredoxin interacting protein (Txnip) protein stability. J Biol Chem 2011;286:29139-45.
Jay SM+, Kurtagic E+, Alvarez LM+, de Picciotto S+, Sanchez E, Hawkins JF, Prince RN, Guerrero Y, Treasure CL, Lee RT*, Griffith LG*. Engineered bivalent ligands to bias ErbB receptor-mediated signaling and phenotypes. J Biol Chem published 2011; 286:27729-40. +*Equal contributors.
Loffredo F*, Steinhauser ML*, Gannon J, Lee RT. Bone-marrow derived cell therapy
stimulates endogenous cardiomyocyte progenitors and promotes cardiac repair. Cell Stem Cell 2011; 8:389-98. *Equal contributors.
Segers VFM, Revin V, Wu W, Qiu H, Yan Z, Lee RT, Sandrasagra A. Protease-resistant Stromal Cell Derived Factor-1 for the Treatment of Experimental Peripheral Arterial Disease. Circulation 2011; 123:1306-15.
Miller RE, Grodzinsky AJ, Cummings K, Plaas AH, Cole AA, Lee RT, Patwari P. Intraarticular injection of heparin-binding insulin-like growth factor 1 sustains delivery of insulin-like growth factor 1 to cartilage through binding to chondroitin sulfate. Arthritis Rheum 2010; 62:3686-94.
Amadi OC, Steinhauser ML, Nishi Y, Chung S, Kamm RD, McMahon AP, Lee RT. A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment. Biomedical Microdevices 2010; 12:1027-41.
Prince RN, Schreiter ER, Zou P, Wiley S, Ting AY, Lee RT, Lauffenburger DA. The heparin-binding domain of HB-EGF mediates localization to cell-cell contact sites and prevents HB-EGF proteolytic release. J Cell Science 2010; 123:2308-18.
Chutkow WA, Birkenfeld AL, Brown JD, Lee HY, Frederick DW, Yoshioka J, Patwari P, Kursawe R, Cushman SW, Shulman GI, Samuel VT, Lee RT. Deletion of the alpha-arrestin protein Txnip in mice promotes adiposity and adipogenesis while preserving insulin sensitivity. Diabetes 2010; 59:1424-34.
Seki K, Sanada S, Kudinova AY, Steinhauser ML, Handa V, Gannon J, Lee RT. Interleukin-33 Prevents Apoptosis and Improves Survival after Experimental Myocardial Infarction through ST2 Signaling. Circulation:Heart Failure 2009; 2:684-91.
Patwari P, Chutkow WA, Cummings K, Verstraeten VL, Lammerding J, Schreiter ER, Lee RT. Thioredoxin-independent regulation of metabolism by the alpha arrestin proteins. J Biol Chem 2009; 284:24996-5003.
Steinhauser ML, Lee RT. Pharmacological Regeneration. Cell Stem Cell 2009; 4:277-8.
Murry CE, Lee RT. Turnover after the Fallout. Science 2009; 324:47-48.
Kakkar R, Lee RT. The IL-33 / ST2 pathway: Therapeutic target and novel biomarker. Nature Reviews Drug Discovery 2008;7:827-40.
Patwari P, Lee RT. Mechanical Control of Tissue Morphogenesis. Circ Res 2008;103:234-43.
Sabatine MS, Morrow DA, Higgins LJ, MacGillivray C, Guo W, Bode C, Rifai N, Cannon CP, Gerszten RE, Lee RT. Complementary Roles for Biomarkers of Biomechanical Strain, ST2 and NT-proBNP, in Patients with ST-Elevation Myocardial Infarction. Circulation 2008;117:1936-44.
Tokunou T, Miller R, Patwari P, Davis ME, Segers VFM, Grodzinsky AJ, Lee RT. Engineering Insulin-like Growth Factor-1 for Local Delivery. FASEB J 2008;22:1886-93.
Segers VFM, Lee RT. Stem Cell Therapy for Cardiac Disease. Nature 2008;451:937-942.
Chutkow WA*, Patwari P*, Yoshioka Y, Lee RT. Txnip Is A Critical Regulator of Hepatic Glucose Production. J Biol Chem 2007; 283:2397-406. *equal contributors
Yoshioka J, Imahashi K, Gabel SA, Chutkow WA, Burds AA, Gannon J, Schulze PC, MacGillivray C, London RE, Murphy E, Lee RT. Targeted Deletion of Thioredoxin-Interacting Protein Regulates Cardiac Dysfunction in Response to Pressure Overload. Circ Res 2007;101:1328-38.
Segers VFM, Tokunou T, Higgins LJ, MacGillivray C, Gannon J, Lee RT. Local Delivery of Protease Resistant SDF-1 for Stem Cell Recruitment after Myocardial Infarction. Circulation 2007;116:1683-92.
Hsieh PCH*, Segers VFM*, Davis ME, MacGillivray C, Gannon J, Molkentin JD, Robbins F, Lee RT. Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes following injury. Nature Medicine 2007; 13:970-974. *equal contributors
Sanada S, Hakuno D, Higgins LJ, Schreiter ER, McKenzie AN, Lee RT. IL-33/ST2 is a Critical Biomechanically-induced and Cardioprotective Signaling System. J Clin Invest 2007;117:1538-1549.